one component substrate (Bethyl)
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one component substrate
One Component Substrate, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+one+component+substrate/TMB+One+Component+Substrate/10__3390_slash_vetsci13040343-91-17-19
Average 94 stars, based on 78 article reviews
One Component Substrate, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+one+component+substrate/TMB+One+Component+Substrate/10__3390_slash_vetsci13040343-91-17-19
Average 94 stars, based on 78 article reviews
one component substrate - by Bioz Stars,
2026-09
94/100 stars
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Enzyme-linked Immunosorbent Assay:Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies Article Snippet: .. A 96-well ELISA microplate (Nunc maxisorp, Thermo Fisher Scientific) was coated with 10 μg/ml of dog IgG1 antibody (A40-120A, Bethyl Laboratories) targeting canine IgG-A and IgG-D or dog IgG2 antibody (A40-121A, Bethyl Laboratories) targeting canine IgG-B, both diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical), the plate was blocked with PBS containing 0.05% Tween20 (Kanto Chemical) and 1% bovine serum albumin (BSA) (Thermo Fisher Scientific) (1% BSA-PBS-T) at 37°C for 1 h. Samples were prepared at 1:2,000 dilution, and added to the plate, followed by incubation at RT for 1 h. The reaction was developed using HRP-conjugated dog IgG1 antibody (A40-120P, Bethyl Laboratories) or HRP-conjugated dog IgG2 (A40-121P, Bethyl Laboratories) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37°C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37 ̊C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: .. Measurement of canine IgGs concentration A 96-well ELISA microplate (Nunc maxisorp, Thermo Fisher Scientific) was coated with 10 μg/ml of dog IgG1 antibody (A40-120A, Bethyl Laboratories) targeting canine IgG-A and IgG-D or dog IgG2 antibody (A40-121A, Bethyl Laboratories) targeting canine IgG-B, both diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical), the plate was blocked with PBS containing 0.05% Tween20 (Kanto Chemical) and 1% bovine serum albumin (BSA) (Thermo Fisher Scientific) (1% BSA-PBS-T) at 37 ̊C for 1 h. Samples were prepared at 1:2,000 dilution, and added to the plate, followed by incubation at RT for 1 h. The reaction was developed using HRP-conjugated dog IgG1 antibody (A40-120P, Bethyl Laboratories) or HRPconjugated dog IgG2 (A40-121P, Bethyl Laboratories) and Incubation:Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies Article Snippet: .. A 96-well ELISA microplate (Nunc maxisorp, Thermo Fisher Scientific) was coated with 10 μg/ml of dog IgG1 antibody (A40-120A, Bethyl Laboratories) targeting canine IgG-A and IgG-D or dog IgG2 antibody (A40-121A, Bethyl Laboratories) targeting canine IgG-B, both diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical), the plate was blocked with PBS containing 0.05% Tween20 (Kanto Chemical) and 1% bovine serum albumin (BSA) (Thermo Fisher Scientific) (1% BSA-PBS-T) at 37°C for 1 h. Samples were prepared at 1:2,000 dilution, and added to the plate, followed by incubation at RT for 1 h. The reaction was developed using HRP-conjugated dog IgG1 antibody (A40-120P, Bethyl Laboratories) or HRP-conjugated dog IgG2 (A40-121P, Bethyl Laboratories) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37°C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37 ̊C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and Article Title: Development of caninized anti-CTLA-4 antibody as salvage combination therapy for anti-PD-L1 refractory tumors in dogs Article Snippet: MaxiSorp Immuno Plates (Thermo Fisher Scientific) were coated with 1 μg/mL CTLA-4 Ig or 10 μg/mL PD-L1 Ig and blocked with SuperBlock T20 (PBS) (Thermo Fisher Scientific). .. Serum samples were incubated, and bound antibodies were detected using HRP-conjugated anti-dog IgG2 antibody (for ca1C5, Bethyl Laboratories) or HRP-conjugated anti-dog IgG1 antibody (for c4G12, Bethyl Laboratories) and Article Title: Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in sheep. Article Snippet: Sheep have been used as a large animal experimental model for studying infectious diseases.. However, due to a lack of staining antibodies and reagents, immunological studies on sheep have not progressed.. The immunoinhibitory receptor programmed death-1 (PD-1) is expressed on T lymphocytes. Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: .. Measurement of canine IgGs concentration A 96-well ELISA microplate (Nunc maxisorp, Thermo Fisher Scientific) was coated with 10 μg/ml of dog IgG1 antibody (A40-120A, Bethyl Laboratories) targeting canine IgG-A and IgG-D or dog IgG2 antibody (A40-121A, Bethyl Laboratories) targeting canine IgG-B, both diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical), the plate was blocked with PBS containing 0.05% Tween20 (Kanto Chemical) and 1% bovine serum albumin (BSA) (Thermo Fisher Scientific) (1% BSA-PBS-T) at 37 ̊C for 1 h. Samples were prepared at 1:2,000 dilution, and added to the plate, followed by incubation at RT for 1 h. The reaction was developed using HRP-conjugated dog IgG1 antibody (A40-120P, Bethyl Laboratories) or HRPconjugated dog IgG2 (A40-121P, Bethyl Laboratories) and Blocking Assay:Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37°C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: The recombinant protein was biotinylated using the Biotinylation Kit/Biotin Conjugation Kit (Fast, Type A)—Lightning-Link (Abcam, Cambridge, UK). .. A 96-well ELISA microplate (Nunc maxisorp; Thermo Fisher Scientific) was coated with 1 μg/ml of each canine IgG subclass diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical, Tokyo, Japan), the plate was blocked with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific) at 37 ̊C for 1 h. Biotinylated r-SpsQ or r-SpA was added to the plate at various concentrations (from 2 ng/ml to 0.5 ng/ml in 2-fold dilution series), followed by incubation at RT for 1 h. The reaction was developed using horseradish peroxidase (HRP)-conjugated NeutrAvidin protein (Thermo Fisher Scientific) and other:Article Title: Anti-PD-L1 antibody Article Snippet: Canine PD-L1-Ig (prepared in 2.4 above) was biotinylated using Lightning-Link Biotin Conjugation Kit (Innova Bioscience) and reacted with various concentrations (0, 2.5, 5 and 10 μg/ml) of rat anti-bovine PD-L1 antibodies 4G12, 5A2 and 6G7 at 37° C. for 30 min, followed by addition to the 96-well plates. Binding Assay:Article Title: Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in sheep. Article Snippet: Sheep have been used as a large animal experimental model for studying infectious diseases.. However, due to a lack of staining antibodies and reagents, immunological studies on sheep have not progressed.. The immunoinhibitory receptor programmed death-1 (PD-1) is expressed on T lymphocytes. Article Title: Development of caninized anti-CTLA-4 antibody as salvage combination therapy for anti-PD-L1 refractory tumors in dogs Article Snippet: Negative controls included the same concentrations of rat IgG 2a , rat IgG 2b (Bio X Cell), and dog IgG (Jackson ImmunoResearch). .. The mixture was added onto the microwell plates, and CTLA-4 Ig binding was detected using NeutrAvidin HRP conjugate (Thermo Fisher Scientific) and Concentration Assay:Article Title: Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. Article Snippet: .. Measurement of canine IgGs concentration A 96-well ELISA microplate (Nunc maxisorp, Thermo Fisher Scientific) was coated with 10 μg/ml of dog IgG1 antibody (A40-120A, Bethyl Laboratories) targeting canine IgG-A and IgG-D or dog IgG2 antibody (A40-121A, Bethyl Laboratories) targeting canine IgG-B, both diluted in PBS at RT for 1 h. After washing with PBS containing 0.05% Tween20 (Kanto Chemical), the plate was blocked with PBS containing 0.05% Tween20 (Kanto Chemical) and 1% bovine serum albumin (BSA) (Thermo Fisher Scientific) (1% BSA-PBS-T) at 37 ̊C for 1 h. Samples were prepared at 1:2,000 dilution, and added to the plate, followed by incubation at RT for 1 h. The reaction was developed using HRP-conjugated dog IgG1 antibody (A40-120P, Bethyl Laboratories) or HRPconjugated dog IgG2 (A40-121P, Bethyl Laboratories) and |
